Selection of novel lipases and esterases for enantioselective biocatalysis

This thesis describes the application of lipolytic enzymes of Bacillus subtilis for the enantiopure production of the chiral intermediate 1,2-O-isopropylidene-sn-glycerol (IPG) out of racemic ester compounds. (+)- IPG is an important chiral synthon in the synthesis of the biologically active form of several well-known pharmaceuticals and endogenous compounds, such as phospholipids, B-adrenoceptor antagonists, and platelet aggregating factors. To improve the yield of enantiopure IPG as compared to production using wild type lipase/ carboxylestererase, we assessed two different strategies to specifically evolve these biocatalysts into high enantioselectivity: paralogous gene analysis and directed evolution using phage display selection. ...

Zie: Chapter 9

	titlepages/contents
	chapter 1
	chapter 2
	chapter 3
	chapter 4
	chapter 5
	chapter 6
	chapter 7
	chapter 8
	chapter 9
	dankwoord
	list of publications
	references
	samenvatting
	thesis

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