The mannitol phosptransferase system of Bacillus stearothermophilus

This thesis describes the mannitol-specific PTS of the thermophilic organism B. stearothermophilus. The mannitol operon of B. stearothermophilus was cloned and the properties of the proteins involved in mannitol uptake and the regulation of the expression of the mtl operon were studied. The operon consists of four genes: mtlA, mtlR, mtlF and mtlD coding for the mannitol transporter IICBmtl, the transcriptional regulator MtrlR, the phosphotransferase IIAmtl and the mannitol-l-phosphate dehydrogenase, respectively. The mtlA, mtlR, and mtlF gene of the mtl operon and the ptsI and ptsH gene of the pts operon, encoding B. stearothemophilys EI and HPr, have been expressed in E. coli and purified ...

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	chapter 1
	chapter 2
	chapter 3
	chapter 4
	chapter 5
	addendum
	references
	samenvatting
	thesis

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