Functional analysis of the integrative plasmid PMEA300 of the actinomycete Amycolatopsis Methanolica.

We are interested in the regulation of primary metabolism in A. methanolica, especially of the pathways for biosynthesis of aromatic amino acids and catabolism of glucose and methanol (De Boeretal., 1989; Dijkhuizen et al,. 1993). Attempts to construct amino acids overproducting strains were hampered by a general lack of methods and tools for genetic studies with A. methanolica. Aim of this work therefore was the construction of A. methanoloca-E. coli shuttle vectors allowing the cloning and molecular characterization of A. methanolica genes encoding enzymes involved in L-phenylalanine biosynthesis.

Zie: Summary and concluding remarks

Summary and concluding remarks

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