Purification and solubilization of muscarinic receptors for the purpose of quantitative receptor assays of anticholinergics

This thesis deals with the optimalization of quantitative receptor assays (QRA) of anticholinergic drugs. QRA have recently emerged as a new tool in bioanalysis, offering challenging perspectives with regard to improving senstitivies and/or selectivities. Similar to immunoassays, QRA is a binding assay in which an anlyte and a (radio)labelled ligand compete for the binding to a spectific protein. ...

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