Neuroendocrine biomarkers in clinical chemistry : method development and clinical validation of automated LC-MS/MS methods

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The aim of this thesis was the development and clinical valida-tion of mass spectrometric methods for the quantification of neuroendocrine biomarkers. The research was focused on
quantification of biogenic amines in the clinical setting as markers the the diagnosis of neuroendocrine tumors. Neuroendocrine tumors secrete neurotransmitters and hormones, especially serotonin and catecholamines in body fluids such as blood. urine and saliva.
Several methods and techniques have been developed in the last decades for the analysis of such compounds. Most of these methods are time-consuming due to extensive manual
sample pretreatment and are susceptible to analytical interference because often conventional non-mass spectrometric detection techniques are applied.
In chapter 2 an overview is given of current methods that employ (automated) liquid chromatography with tandem mass spectrometric detection (LC-MS/MS) for the
quantification of low-molecular endogenous compounds. Special attention is paid to the analysis of biogenic amines arid their metabolites.

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