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Microbial dynamics in subgingival biofilms

(2010) Zijnge, Vincent

Periodontal diseases are the most common presentation of oral pathology and affect the tooth supporting tissues, including the cementum, the periodontal ligament, the alveolar bone and the gingival lining. Severe forms of periodontitis affect 10-15% of the adult population and result in the loss of teeth when left untreated. The aetiology of periodontitis is complex and is the result of bacterial accumulations on the tooth surface, compromising host factors like gene-polymorphisms or medical disorders like diabetes and modified by life style factors such as smoking. A model that describes how the different factors involved in periodontal pathogenesis interact is described in Chapter 1. Periodontitis starts with the accumulation of bacteria on the tooth surface where they form biofilms. To study the role of these biofilms in periodontitis, molecular techniques are developed and optimized for oral bacteria. In Chapter 2 Denaturing Gradient Gel Electrophoresis (DGGE) is introduced in oral microbiology. DGGE fingerprints of complex microbial communities are used to study shifts in the population after treatment and Exiguobacterium aurantiacum was identified in 13 out of 25 samples. It was possible to detect the most relevant periodontal pathogens in DGGE profiles to a level comparable with culturing and species-specific PCR ...

Zie: Chapter 3

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