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Detection of DNA hypermethylation as a diagnostic tool in cervical neoplasma tool in cervical neoplasia

(2010) Yang, Nan

Current cytomorphological based cervical cancer screening has some limitations due to relatively high false positive and false negative screening results. New technologies have been introduced to improve the current screening approach.
Different techniques to detect hypermethylation are reviewed in chapter 2. In chapter 3 to evaluate whether gene promoter methylation can be used to distinguish LSIL from HSIL lesions, DNA of paraffin embedded tissues from normal cervix (n=20), LSIL (n=20), HSIL (n=20), adenocarcinomas (AC) (n=20) and squamous cell cervical cancers (SCC) (n=40) was studied first because histology of the tissue is still considered as the golden standard. QMSP of the same nine genes was performed to determine whether the methylation status of the underlying lesion was reflected in (55 available) corresponding cervical scrapings. In chapter 4, a more in-depth analysis of the methylation patterns of these 13 candidate genes in cervical cancer and normal tissue specimens was performed. Their possible relevance for the early detection of cervical cancer was evaluated in a large series of scrapings from patients with cervical cancer, low- and high-grade CIN and from otherwise healthy women. In chapter 5, we used QMSP to determine differences in gene promoter methylation in paired lesions from patients with early and late recurrent HSIL. In chapter 6 a feasibility study for the detection of DNA hypermethylation by QMSP in cervico-vaginal samples collected by a novel self-sampling device was performed. Methylation status, cytomorphology and hr-HPV positivity of cervico-vaginal samples obtained by a novel self-sampling device were analyzed.

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	Hoofdstuk 1
	Hoofdstuk 2
	Hoofdstuk 3
	Hoofdstuk 4
	Hoofdstuk 5
	Hoofdstuk 6
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