Slow microfiltration or slow microdialysis to versatile biomonitoring

Important in human and animal research is the methodology to monitor stress without interference with the normal stress physiology. Related to this issue is or how to collect biological samples from an intact organism without stress, and how to store and analyse biochemical and pharmaceutical compounds. The present thesis is focused on the development of continuous monitoring devices in the freely moving subject that can be applied to a wide variety of compounds, including the glucocorticoids corticosterone and/or cortisol. The present methodologies to monitor pharmaceutical compounds and various brain energy metabolites illustrate their versatility and their usefulness. To explore the temporal patterns of peptides, drugs, metabolites or hormones, conventionally multiple samples have to be collected manually which is often stressful and invasive. Stress is often an unwanted source of experimental error, because stress affects both the physiology and metabolism, thus interfering with or even impairing subtle experimental designs. This may add to between-animal variations and eventually leading to inconsistencies of experimental procedures and results. To reduce stress and release of stress-related hormones, there is a need to refine animal experimental procedures, thus avoiding handling, restraining and bio-sampling. To reduce experimental stress in animals we propose and explored two sampling techniques: microfiltration and MetaQuant microdialysis.

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